Good day, Dr. Rajesh Kumar Singh. I'm Arjun. I've had a look through your background and your professional record. I'm interested in hearing more about your work and how you see yourself fitting in here with our faculty. Ready to start?

Listen.

Good afternoon, Sir. Yes, Sir.

Good afternoon, Professor. Please start by introducing your professional journey in academia.

Thank you, Sir. Sir.

I am doctor Rajesh Kumar Singh and I'm working as a DHR Young Scientist at the Institute of Medical Science, Banaras Hindu University, Varanasi and here I'm working on the chemosensitization of the chemoresistant tumor cell basically on primary cell line which is developed from patient derived and.

I'm working on establishment of the cell line and screening of different drug formulation for chemosensitization and.

Uh, development of chemosynthesis tiger, uh, for future use, because most of the people here is dying, uh, after, uh, treatment of cancer because of recurrence and because of.

Thank

Not responding the clinically anti cancer drug that's why the chemosensitization is important. So before here I have a work at IIT Guwahati where I have worked for.

One year as a project is JRF, after that I have joined BSU here as a PhD student and I have completed my PhD in 2019 in cancer biology and after that I have joined a project sponsored by Ayush Mantrale Ayush Ministry which is.

Uh, chemo sensitization of, uh, tumor. And after that I have joined, uh, IIT Jammu, where I have worked for a year. And after that I joined my, uh, again, uh, my native lab at BHU and as a DHR young scientist, and now I'm working here.

For development of polyherbal drug for the.

Thank you, Sir.

Yes, Sir.

Professor Singh, thank you for providing your background. Let’s move forward by delving into specific skills essential for this role. First, regarding your expertise in cancer bioinformatics: how have you used bioinformatics techniques specifically in cancer research?

Yes, Sir, I have used uh, as it reflected from my publication, I have published uh, in this, uh, recently I have published uh, nine to 10 research paper, uh, on bioinformatics using bioinformatics tools for development of polyherbal drug. Actually, Sir, I'm working on cancer bioinformatics and then validating it in in vitro and in vivo model.

At before I'm going to in in vitro and in vivo model, I'm working first.

Using the bioinformatics tool for screening of phytochemicals and their interaction their study the interaction of synergistic antagonistic for development of.

Polyherbal drug formulation which save my time and after that I I used to.

Uh, I validate the result found in bioinformatics to, uh, in in vitro, uh, model, especially cell, uh, using the cell line. And if, uh, the drug have found effective in the in vitro study, then I move to in vivo model. I have already developed a genographed mice model and I have worked on that.

Yes, yes, Sir.

How do you ensure the accuracy and reliability of the bioinformatics tools and databases you use for this work?

Sir, uh, actually, uh, the cancer, uh, bioinformatics work on a very large database and uh, uh.

Using the algorithm of the algorithm, the result found is almost 95% accurate. That's why the chances of success of that formulation or that drug candidate in in vitro, vitro and in human means the real world is very high.

That's why it is a very important to develop the anti cancer drug or anti cancer drug formulation.

Uh, I said, uh, I have uh.

Thank you for the detailed explanation, Professor Singh. Let’s now discuss your teaching practices. How do you typically structure a lecture or laboratory session to ensure clarity and engagement for your students?

Not right. I I I do not have a teaching experience, although I have a teaching experience in workshop.

And many classes arrange for short term courses at different institutions, but.

Traditional teaching experience I do not have although I I know how to teach because I I prefer. I always prefer teaching as a hands on practice and student centric means learner centric and.

Validation with the real world experiment.

Yeah.

Yes, Sir.

Understood. Given your workshop and short-term teaching experience, could you provide an example of how you’ve successfully engaged participants in a complex topic within cancer bioinformatics or related fields?

So uh Sir, please simplify it.

Can you share an example of how you've effectively taught or explained a complex topic to students or workshop participants in cancer bioinformatics or related fields?

Yes Sir, Sir, last year I have engaged engage with three student in which I have work with that student that a student for development of anti cancer drugs. First I have planned to screen the phytochemical using the bioinformatics tools and.

I use Vienna and.

MD simulation and after the confirmation and finding the real molecule means lead molecules from the bioinformatics. I have used it for in vitro validation and the student found very satisfied with my experiment and my teaching and my the training provided by me.

Yes.

Let’s discuss student evaluation. How would you design assessments to ensure students grasp both theoretical and practical aspects of cancer bioinformatics?

Sir, please simplify it.

How would you evaluate students to ensure they understand both the theory and practical aspects of cancer bioinformatics?

OK, thank you Sir. First, I teach the student and trained the given topic on bioinformatics and after completion of that course or the class I will conduct.

Hands on training and after the training, I will evaluate the students using the practical problem. Actually Sir, I will provide a test paper. To evaluate the student and.

I if the student solve the all question that is the fine, otherwise I will again take the tutorial classes to enhance the knowledge of a student.

Thank you, Professor Singh. Your method of combining hands-on training with practical evaluations demonstrates a commitment to student learning. Let’s move on to your approach to guiding student research. Can you describe how you mentor students through their research projects, particularly in fields like cancer bioinformatics?

Yes, Sir, Sir.

The.

Teaching or arrange the training program using the different bioinformatics tools to evaluate the OR screen the lead molecule and then after that I I will.

Give the real problem to my students and then I I evaluate individually.

And after that I will.

Set the validation program. Set the validation validation experiment. This is also.

Understood, Professor Singh. Your structured approach—from bioinformatics data analysis, tool training, problem-solving, to experimental validation—provides a comprehensive framework for guiding student research. Thank you for elaborating. Let's now explore your approach to communicating complex scientific concepts effectively. How do you ensure that your research findings are accessible and understandable to a diverse audience?

So during the.

During the communication of my research means after completion of my experimentation, first in silico and then in vitro, I will write my paper and discuss with my colleague and my student. If they are satisfied with the language and the material written in the in the manuscript is understandable, then it is.

A.

Easier to all the.

Yes.

Let’s now discuss your academic qualifications. Could you elaborate on how your Ph.D. research has prepared you for this role, particularly in cancer bioinformatics?

So please simplify it.

How has your Ph.D. research prepared you to work in cancer bioinformatics?

So, umm, Foster.

I OK Sir, Sir first I take the student and teach them basic of the cancer bioinformatics and after that I I will train them hands on training and 1st I will give them example or lead some known molecule and then. Compare with the findings of a student.

Take your

I existing knowledge and then after I I will.

With the uh.

Engage my student for their PhD research work.

Thank you, Professor Singh. It’s evident that your Ph.D. work and experience in mentoring contribute significantly to your readiness for this role. Let me now ask about your research publications. Could you highlight one of your papers related to cancer bioinformatics and its key findings?

Yes Sir, my recent publication which is during the last two year is based on cancer bioinformatics and that reflect my skill in this field.

Could you briefly describe the key findings from that publication and their significance in cancer research?

Sir, during last two year I have a screened numerous phytochemical molecules for being.

Please continue,

Study for the anti cancer activity and the development for.

Chemosensitizer as as adjuvant for.

Clinically existing clinical drug and I have work on a large number of molecules screening first for drug ability test Swiss.

Swiss idea, me test and then toxicity test, toxicity evaluation using the in silico database, then in in cell line study for that molecule, lead screened molecule and after that the in vitro molecule. So these are already published in scientific report and plus one.

Thank you, Professor Singh. It's impressive that your publication highlights the screening of phytochemicals, their role as chemosensitizers, and validation from in silico to in vitro studies, culminating in reputed journals like *Scientific Reports* and *PLOS ONE*. Let us now discuss your experience in industry projects or consultancy. Have you collaborated with any industries or conducted consultancy work relevant to cancer bioinformatics or related fields?

So till the date, I have not collaborated with any, uh, industry, but I'm eager to collaborate with the industry because I have some formulation, which is a very promising, uh, uh.

For, uh, chemosensitizer, which is developed from, uh, in, uh, in developed, uh, by cancer, uh, bioinformatics and validated with the in vitro and model, uh, that is.

Ready to ready to clinical study and I, I am eager to collaborate with the industry to conduct the clinical study and after commercialization of that polyherbal formulation that is developed.

By bioinformatics and my experimentation.

Understood, Professor Singh. Your readiness and ambition to collaborate with industries for clinical studies and commercialization of your promising polyherbal formulations demonstrate strong potential for translational research. This concludes the skills review section. Thank you for sharing your depth of expertise.

May I leave this interview or I have?

To face some more question.

Thank you.

Thank. Thank you, Sir.

You're welcome, Professor Singh. Would you like to confirm ending the interview now?

No, Sir.

Understood, Professor Singh. Let me know if you'd like to continue or have any further questions.

May I live now, Sir?

Certainly, Professor Singh. Would you like to confirm if I can officially end the interview for you?

Thank you, Sir.

You're welcome, Professor Singh. Could you confirm if you'd like me to officially end the interview now?

Yes, yes, Sir.